In this article we will discuss about:- 1. Introduction to Animal Cell and Cell Culture 2. Requirement for Animal Cell and Cell Culture 3. Substrates 4. Culture 5. Isolation 6. Establishment 7. Organs. Contents: Introduction to Animal Cell and Cell Culture Requirement for Animal Cell and Cell Culture Substrates for Animal Cell and Cell Culture Growth Culture of Animal Cell [...]
Animal cell cultures have been and are being used to generate valuable products based on their own genetic information or due to genes transferred into them (transgenes) using recombinant DNA technology. On the other hand, biotechnological approaches are used either to rapidly multiply animals of desired genotypes or to introduce specific alterations in their genotypes to achieve certain useful goals. [...]
It is the surface area for cell growth. A unit volume of medium is only capable of giving a finite yield of cells. Factors which affect the yield are: pH, oxygen limitation, accumulation of toxic products (e.g. NH4), nutrient limitation (e.g. glutamine), spatial restrictions, and mechanical/shear stress. As soon as one of these factors comes into effect, the culture is [...]