In Balanoglossus, there is no definite endoskeleton but there are four structures of a supporting nature, they are a buccal diverticulum, proboscis skeleton, branchial skeleton, and a pygochord.

1. Buccal Diverticulum:

Buccal diverticulum is a hollow pre-oral outgrowth, extending from the roof of the buccal cavity into the proboscis. It was for a long time also called a notochord or a stomochord on the assumption that it represents the anterior portion of the notochord of chordates.

The buccal diverticulum extends forward in some enteropneusts as a slender vermiform process or appendix. The buccal diverticulum is neither analogous nor homologous with the chordate notochord; histologically it is identical with the wall of the buccal cavity, it is apparently nothing more than a pre-oral extension of the gut.

Skeletal Elements

2. Proboscis Skeleton:

Proboscis skeleton or nuchal skeleton is formed by the basement membrane becoming thick to form a laminated plate from which arise two thin horns or cornua, the plate usually has a midventral keel. The proboscis skeleton lies in the proboscis stalk, while its horns extend into the roof of the buccal cavity.

3. Branchial Skeleton:

The walls of the U-shaped gill-clefts are supported by skeletal rods called primary and secondary gill-rods formed by thickening of the basement membrane.

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4. Pygochord:

Pygochord is longitudinal rod-like structure extending from the ventral side of the intestine to the body wall, its function is not known but it may support the soft abdominal region.